Journal: medRxiv

Article Title: Elevated plasma Complement Factor H Regulating Protein 5 is associated with venous thromboembolism and COVID-19 severity

doi: 10.1101/2022.04.20.22274046

Figure Lengend Snippet: Platelet activation was measured by surface expression of ( A ) P-selectin, ( B ) activated GP IIb/IIIa or ( C ) CD63, following treatment of platelet rich plasma with different concentrations of ( i ) adenosine diphosphate (ADP) ( ii ) convulxin or ( iii ) TRAP6, following pre-incubation (10 minutes) with recombinant CFHR5, or PBS control. Each experiment is represented by an individual point and paired experiments connected by a dotted line. *p<0.05 **p<0.01 ***p<0.001 (p for trend bottom right).

Article Snippet: Platelet-rich plasma (PRP) or isolated platelets were incubated with recombinant CFHR5 in PBS (6 μg/ml, 3845-F5, R&D systems) or PBS alone for 10 minutes before treatment with varying concentrations of ADP (1-5 μM), TRAP-6 (3-15 μM) or convluxin (1-6 ng/ml) for 15 minutes.

Techniques: Activation Assay, Expressing, Clinical Proteomics, Incubation, Recombinant, Control

Journal: Stem cells (Dayton, Ohio)

Article Title: A Glycovariant of Human CD44 is Characteristically Expressed on Human Mesenchymal Stem Cells

doi: 10.1002/stem.2549

Figure Lengend Snippet: Immunophenotypic profile of surface antigens on hMSCs derived from bone marrow (BM-hMSCs) or adipose tissue (A-hMSCs)

Article Snippet: For flow cytometry measurement of E-selectin ligand expression, untreated and FTVII-treated hMSCs were stained in a 3-step procedure using recombinant mouse E-selectin–human Ig chimera (0.5 μg/ml; R&D Systems) in Ca 2+ -containing binding buffer (HBSS, 5mM HEPES, 2mM CaCl 2 and 5%FBS), followed by staining with rat anti-mouse E-selectin (CD62E) mAb (R&D Systems), and then FITC-conjugated goat anti-rat IgG (Southern Biotech), each for 30 min at 4°C.

Techniques: Derivative Assay